With a better understanding of the HSC microenvironment and the ability to precisely recapitulate its components, we may be able to gain control of HSC behavior. In this work we developed a novel, biomimetic PEG hydrogel material as a substrate for this purpose and tested its potential with an anchorage-independent hematopoietic cell line, 32D clone 3 cells.
To evaluate the system's capabilities, we observed the effects of the biomolecules on 32D cell adhesion and morphology. We demonstrated that the incorporation of RGDS onto the surfaces promotes 32D cell adhesion in a dose-dependent fashion.
By recapitulating aspects of the HSC microenvironment using a PEG hydrogel scaffold, we have shown the ability to control the adhesion and spreading of the 32D cells and demonstrated the potential of the system for the culture of primary hematopoietic cell populations. Published by Elsevier Ltd. Small-diameter vascular grafts made of biomedical polytetrafluoroethylene PTFE suffer from the poor long-term patency rate originating from thrombosis and intimal hyperplasia, which can be ascribed to the insufficient endothelialization and chronic inflammation of the materials.
Hence, bio-functionalization of PTFE grafts is highly desirable to circumvent these disadvantages. In this study, a versatile "implantation-incubation" approach in which the biomedical PTFE is initially modified by plasma immersion ion implantation PIII is described. After the N 2 PIII treatment, the surface of biomedical PTFE is roughened with nanostructures and more importantly, the abundant free radicals generated underneath the surface continuously migrate to the surface and react with environmental molecules.
Taking advantage of this mechanism, various biomolecules with different functions can be steadily immobilized on the surface of PTFE by simple solution immersion. In addition to retaining the bioactivity, the surface-functionalized PTFE exhibits reduced thrombogenicity, facilitates the recruitment of endothelial progenitor cells, and even alleviates the inflammatory immune responses of monocytes-macrophages and is thus promising to the development of small-diameter prosthetic vascular grafts with good long-term patency. In addition to its high stability, reproducibility and relatively long lifetime, the proposed optical sensor revealed good selectivity for target ions over a large number of alkali, alkaline earth, transition, and heavy metal ions.
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The proposed optical membrane displays linear responses from 1. The limits of detection LOD were 2. For this, a novel method was developed in this work by co- immobilization of Ln and heparin complex on poly-L-lysine modified Ti surface. Take the advantage of the specific interaction between Ln and heparin, Ln and heparin complexes with different concentration ratios were set up for creating different exposure density of these two types of biomolecules. Furthermore, the adherent density and proliferation activity of ECs and EPCs were positively correlated with Ln concentration.
According to the existing results, this study demonstrated that in a certain range of heparin and laminin concentration ratio. Penicillium subrubescens, a new species efficiently producing inulinase. Inulinases hydrolyse inulin To find efficient inulin degrading fungi, fungal strains from the Fungal Biotechnology Culture Collection FBCC at University of Helsinki and 74 freshly isolated strains from soil around Jerusalem artichoke tubers were screened in liquid cultures with inulin as a sole source Inulinase and invertase activities were assayed by the dinitrosalicylic acid DNS method and a freshly isolated Penicillium strain originating from agricultural soil FBCC was the most efficient inulinase Mansouri, S.
Inulinases hydrolyse inulin into. Application of agave subproducts for production of microbial inulinases. Full Text Available Mexico is the center of origin of the genus Agave spp. During tequila production, a huge amount of waste is produced, including leaves that are not used. Main compounds found in these subproducts are polysaccharides, specifically inulin.
Current review focuses on the use of these agricultural wastes to obtain inulinase production, and in the production of high fructose syrup using these enzymes in these wastes. Currently, high fructose syrups, especially oligosaccharides, have prompted much interest for their benefits to health due to its low glycemic index and their function as prebiotic in the intestinal flora.
The main microorganisms involved with inulinases production belong to the genera Aspergillus and Kluyveromyces. This review emphasizes on the advances achieved in the production of inulinases by yeast K. Enhanced production of extracellular inulinase by the yeast Kluyveromyces marxianus in xylose catabolic state. The production of extracellular proteins by the thermotolerant yeast Kluyveromyces marxianus, which utilizes various sugars, was investigated using media containing sugars such as glucose, galactose, and xylose. SDS-PAGE analysis of culture supernatants revealed abundant production of an extracellular protein when cells were grown in xylose medium.
The N-terminal sequence of the extracellular protein was identical to a part of the inulinase encoded by INU1 in the genome.
Disruption of INU1 in the strain DMKU lost the production of the extracellular protein and resulted in growth defect in sucrose and inulin media, indicating that the extracellular protein was inulinase sucrase. In addition, six K. However, expression analysis indicated that the INU1 promoter activity was lower in the xylose medium than in the glucose medium, suggesting that enhanced production of inulinase is controlled in a post-transcriptional manner. The production of inulinase was also higher in cultures with more agitation, suggesting that oxygen supply affects the production of inulinase.
Taken together, these results suggest that both xylose and oxygen supply shift cellular metabolism to enhance the production of extracellular inulinase. Published by Elsevier B. Inulinase production in a packed bed reactor by solid state fermentation. In this work, production of inulinase was carried out in a packed bed reactor PBR under solid state fermentation.
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Kluyveromyces marxianus var. The parameters like air flow rate, packing density and particle size were optimized using response surface methodology RSM to maximize the inulinase production. The optimum conditions for the maximum inulinase production were: air flow rate - 0. At these optimized conditions, the production of inulinase was found to be Tyrosinase EC 1. The homogeneity of the chitosan-gelatin biocomposite film was characterized by X-ray diffraction analyses.
To evaluate immobilization efficiency, chitosan-gelatin-Tyr bio-composite films were analyzed by field emission scanning electron microscopy, atomic force microscopy and UV-spectroscopy. The rough morphology of the film led to a high loading of enzyme and it could retain its bioactivity for a longer period. Moreover, the immobilized enzyme exhibited broader pH and temperature profile as compared to free counterpart.
Immobilized enzyme was further evaluated for the synthesis of L-DOPA 2,4-dihydroxy phenylalanine which is a precursor of dopamine and a potent drug for the treatment of Parkinson's disease and for myocardium neurogenic injury. Yarrowia lipolytica is a promising platform for the single cell oil SCO production.
However, the inulinase activity of a transformant X2 carrying a single exo- inulinase gene was only These results demonstrated that the co-expression of the exo- and endo- inulinase genes significantly enhanced the SCO production from inulin due to the improvement of the inulinase activity and the synergistic action of exo- and endo- inulinase.
Besides, over Inu2 and Inu3 had the same pH optimum at 5. Inu2 and Inu3 had low km values 0. From these findings, R. Purification and Characterization of Exo- Inulinase from Paenibacillus sp. This study intended to purify and characterise exo- inulinase of diesel-degrading Paenibacillus sp. The whole genome sequencing of Paenibacillus sp. This isolate was capable of producing a maximum of An exo- inulinase of Catalytic constant, k cat was calculated to be The results of amino acid alignment and the complete degradation of inulin into fructose by the purified enzyme confirmed that inulinase from Paenibacillus sp.
D9 is an exo-form. The phylogenetic tree based on the protein sequences indicates that bacterial exo- inulinases possess a common ancestry. The purpose of this study was to develop a cheaper and stable biostrip for investigation of TG levels in serum. Methods: A reusable enzyme-strip was prepared for TG determination by co- immobilizing lipase, glycerol kinase GK, glycerolphosphate oxidase GPO and peroxidase HRP directly onto plasticized polyvinyl chloride PVC strip through glutaraldehyde coupling.
The method was evaluated by studying its recovery, precision and reusability. Results: The enzyme-strip showed optimum activity at pH 7.
The strip was used for determination of serum TG. The detection limit of the method was 0. Analytical recovery of added triolein was 96 per cent.
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Within and between batch coefficients of variation CV were 2. The strip lost 50 per cent of its initial activity after its uses during the span of days, when stored at 4 o C.
The strip provided reuses of enzymes with only 50 per cent loss of its initial activity. The method could be used for preparation of other enzyme strips also. Effects of carbon sources, oxygenation and ethanol on the production of inulinase by Kluyveromyces marxianus YX Full Text Available Inulinase is one of the most important factors in consolidated bioprocessing, which combines enzyme production, inulin saccharification, and ethanol fermentation into a single process. In our study, inulinase production and cell growth of Kluyveromyces marxianus YX01 under different conditions were studied.
Carbon source was shown to be significant on the production of inulinase , because the activity of inulinase was higher using inulin as a carbon source compared with glucose or fructose. The concentration of the carbon source had a repressive effect on the activity of inulinase.
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Enzyme activity was also strongly influenced by aeration rate. It has been shown that the activity of inulinase and cell growth under anaerobic conditions were maintained at low levels, but aeration at 1. Inulinase activity per unit biomass was not significantly different under different aeration rates.
Ethanol had a repressive effect on the cell growth. Magnetite nanoparticles coated with covalently immobilized ionic liquids as a sorbent for extraction of non-steroidal anti-inflammatory drugs from biological fluids. The NPs were characterized via FTIR and scanning electron microscopy and evaluated with respect to the extraction of the nonsteroidal anti-inflammatory drugs NSAIDs tolmetin, indometacin and naproxen from blood samples. Supercritical fluid extraction was used to eliminate matrix effects before extraction with the mag-NPs.
The effects of pH value of sample solution, amount of adsorbent, times of adsorption and desorption, salt effect, type and volume of suitable solvent for desorption were optimized. Under optimum conditions, magnetic solid phase extraction MSPE resulted in limits of detection that range between 0. Full Text Available Summary.